Lab 3: Chromatography Lab

   The purpose of the Chromatography Lab is to reproduce a multicolor are pattern with a variety of black pens using paper chromatography. The experiment was started by drawing designs with pens on a piece of filter paper. A wick was created using another piece of paper which pierced the filter paper. The wick and filter paper were then placed into a cup of water. Slowly the water spread throughout the filter paper creating the design.

1. Why is it important that only the wick and not the filter paper circle be in contact with the water in the cup? 
   It is important the only the wick is in contact of the water to ensure that the water spreads across the filter paper and through the black ink.

2. What are some of the variables that will affect the pattern of colors produced in the filter paper?
   The variables that will affect the pattern of colors produced in the filter paper are the initial ink design and the black ink from the pen.

3. Why does each ink separate into different pigment bands?
   The ink separates into different bands because it is made up of different compounds. When the water passes through the ink compounds, the compounds dissolve and separate into different bands.

4. Choose one color (e.g., yellow, red, or blue) that is present in more than one type of ink. Is the pigment (compound) that gives this color always the same? Do any of the pens appear to contain common pigments? Explain.
   A blue pigment is present in all of the filter paper designs. The pigment that gives this color is the same, but there are different amounts of it. Many pens contain the blue pigment because blue is in all of the designs.

5. Why are only water-soluble markers or pens used in this activity? How could the experiment be modified to separate the pigments in "permanent" markers or pens?
   Water-soluble markers are used because permanent marker's pigments will not spread across the filter paper. The experiment could be modified to separate the pigment by using other chemicals besides ink.





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